Compared with oligo(dT) primers, these primers are certainly not template unique and so are suited to DNA synthesis employing Klenow fragments with DNA templates or for cDNA synthesis utilizing reverse transcriptase with mRNA templates.

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A maximum of twenty assembly accessions are permitted. FASTA sequences are restricted to 300M. Notice which the organism subject is dismissed for tailor made databases. Enter sequence accession, FASTA sequence or assembly accession

Swimming pools of pre-designed primers check here may help generate in depth expression profiles, Primarily wherever assay sensitivity is critically vital.

In distinction to random primers, oligo (dT) primers aren't well suited for degraded samples but are fantastic for developing cDNA libraries and recommended for use with reverse transcriptases (RTs) as Superscript IV RT.

Assist This controls if the primer should span an exon junction on your own mRNA template. The option "Primer ought to span an exon-exon junction" will direct This system to return a minimum of one primer (in just a provided primer pair) that spans click here an exon-exon junction.

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